Jim Dabrowiak and Jerry Goodisman
Syracuse University
Department of Chemistry

TOP - We're interested in mapping the folded conformations of RNA using techniques such as quantitative footprinting. The major packaging domain of HIV-1 may fold in a manner similar to the low free energy form predicted by the Borer group.

BOTTOM - The gel pattern shows how the RNase T1 cutting pattern changes upon increasing the concentration of paromomycin. The cleavage pattern helps us to evaluate alternative folds.